| 质粒名称: | PCS2+ Cas9 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 122948 |
| 存储实验室: | Janet Rossant |
|
载体骨架基本信息 |
|
| 载体名称: | PCS2+ |
| 完整质粒大小: | 8355 bp |
| 载体类型: | Synthetic Biology细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Cas9 |
| 物种来源: | Synthetic |
| 启动子: | SP6 |
| 克隆方法: | Gibson Cloning |
| 5’克隆位点: | AgeI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | ATT TAG GTG ACA CTA TAG |
| 3’测序引物 | GGA TAA CAA TTT CAC ACA GG |
|
文献引用方法 |
|
| 材料和方法部分: | PCS2+ Cas9 was a gift from Janet Rossant (Addgene plasmid # 122948 ; http://n2t.net/addgene:122948 ; RRID:Addgene_122948) |
| 参考文献部分: | Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Gu B, Posfai E, Rossant J. Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. 10.1038/nbt.4166 PubMed 29889212 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS PCS2+ Cas9 8355 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION For in vitro transcription to produce Cas9 mRNA for microinjection
into mouse embryos.
ACCESSION .
VERSION .
KEYWORDS PCS2+ Cas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8355)
AUTHORS Gu B, Posfai E, Rossant J
TITLE Efficient generation of targeted large insertions by microinjection
into two-cell-stage mouse embryos.
JOURNAL Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166.
PUBMED 29889212
REFERENCE 2 (bases 1 to 8355)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8355
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1..66
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 73..93
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 118..4218
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 4219..4266
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
promoter complement(4298..4315)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 4320..4454
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4344..4363)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 4398..4417
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(4567..4587)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(4567..4585)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4606..4622)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4606..4622)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4619..4641)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4630..4646
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4654..4684)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4699..4720
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4837..4854)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5008..5596)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5088..5107)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5767..6627)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6390..6409
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6628..6732)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6758..7213)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6895..6916)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7107..7126
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 7291..8268
/label=CMV IE94 promoter
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
promoter 8303..8321
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 8303..8320
/label=SP6
/note="SP6 promoter, forward primer"
ORIGIN
1 gactataagg accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac
61 gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac
121 aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc
181 gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc
241 atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc
301 cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg
361 caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa
421 gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc
481 gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg
541 gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc
601 aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac
661 aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac
721 gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg
781 gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt
841 gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc
901 aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc
961 ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg
1021 agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc
1081 aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag
1141 ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac
1201 attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag
1261 atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag
1321 cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt
1381 ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag
1441 atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc
1501 gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg
1561 gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg
1621 cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac
1681 gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc
1741 gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag
1801 cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc
1861 gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag
1921 gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc
1981 ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg
2041 ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg
2101 agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc
2161 ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg
2221 acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag
2281 cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag
2341 gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa
2401 atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag
2461 cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa
2521 aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg
2581 tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg
2641 cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag
2701 aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac
2761 tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag
2821 gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg
2881 gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag
2941 tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg
3001 gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac
3061 cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct
3121 aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc
3181 gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc
3241 atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg
3301 atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc
3361 gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca
3421 ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga
3481 aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct
3541 gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag
3601 ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg
3661 gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc
3721 ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag
3781 ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat
3841 gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac
3901 aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg
3961 gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc
4021 agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc
4081 gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg
4141 ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg
4201 tctcagctgg gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag
4261 aaaaagtaag aattcaaggc ctctcgagcc tctagaacta tagtgagtcg tattacgtag
4321 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
4381 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
4441 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
4501 tgtgggaggt tttttaattc gcggccgcgg cgccaatgca ttgggcccgg tacccagctt
4561 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
4621 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggga gcataaagtg
4681 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
4741 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
4801 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
4861 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
4921 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
4981 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
5041 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
5101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
5161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
5221 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
5281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
5341 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
5401 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
5461 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
5521 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
5581 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
5641 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
5701 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
5761 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
5821 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
5881 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
5941 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
6001 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
6061 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
6121 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
6181 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
6241 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
6301 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
6361 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
6421 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
6481 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
6541 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
6601 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
6661 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
6721 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
6781 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
6841 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
6901 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
6961 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
7021 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
7081 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
7141 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
7201 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
7261 cgggcctctt cgctattacg ccagtcgacc gccaattcaa tatggcgtat atggactcat
7321 gccaattcaa tatggtggat ctggacctgt gccaattcaa tatggcgtat atggactcgt
7381 gccaattcaa tatggtggat ctggacccca gccaattcaa tatggcggac ttggcaccat
7441 gccaattcaa tatggcggac ctggcactgt gccaactggg gaggggtcta cttggcacgg
7501 tgccaagttt gaggaggggt cttggccctg tgccaagtcc gccatattga attggcatgg
7561 tgccaataat ggcggccata ttggctatat gccaggatca atatataggc aatatccaat
7621 atggccctat gccaatatgg ctattggcca ggttcaatac tatgtattgg ccctatgcca
7681 tatagtattc catatatggg ttttcctatt gacgtagata gcccctccca atgggcggtc
7741 ccatatacca tatatggggc ttcctaatac cgcccatagc cactccccca ttgacgtcaa
7801 tggtctctat atatggtctt tcctattgac gtcatatggg cggtcctatt gacgtatatg
7861 gcgcctcccc cattgacgtc aattacggta aatggcccgc ctggctcaat gcccattgac
7921 gtcaatagga ccacccacca ttgacgtcaa tgggatggct cattgcccat tcatatccgt
7981 tctcacgccc cctattgacg tcaatgacgg taaatggccc acttggcagt acatcaatat
8041 ctattaatag taacttggca agtacattac tattggaagt acgccagggt acattggcag
8101 tactcccatt gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt acatccccat
8161 tgacgtcaat ggggaggggc aatgacgcaa atgggcgttc cattgacgta aatgggcggt
8221 aggcgtgcct aatgggaggt ctatataagc aatgctcgtt tagggaaccg ccattctgcc
8281 tggggacgtc ggagcaagct tgatttaggt gacactatag aatacaagct acttgttctt
8341 tttgcaggat ccatg
//
